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171.
The rate of recovery of invertebrate communities following habitat restoration has received little attention, despite the importance of invertebrates in ecosystem dynamics. In experimental trials in a mined peat bog in New Zealand, we compared the short-term rate of beetle community re-assembly at sites restored using management techniques varying in cost and effort to implement, and subsequently examined the long-term rate of beetle community convergence towards the ‘target’ community structure of an undisturbed peat bog. There was a direct relationship between the rate of beetle community re-assembly and the cost and effort applied to plant community restoration treatments (processed peat with no seed, processed peat with seed, and direct habitat translocation). To test the longer term rate of beetle community convergence following habitat restoration, we monitored beetle assemblages on a chronosequence of experimental habitat islands in which restoration was initiated 1, 13, 24, 25, 42 and 72 months previously. With increasing age, the plant community became more diverse and structurally complex, and beetle community composition converged rapidly on the target community structure of an undisturbed peat bog. Using a simple linear regression analysis on ordination axis scores, we obtained the quantitative prediction that the beetle community on restored islands would converge on the average community composition of an undisturbed peat bog within just 7-8.5 years. Our results clearly show that rapid invertebrate community re-assembly can occur in direct response to management techniques focused on establishing vegetation structure. Even the use of a low-cost restoration technique (processed peat with seed added) was effective in initiating remarkably rapid invertebrate community re-assembly in cutover peat bogs within 10 years.  相似文献   
172.
The use of anabolic substances is prohibited in food-producing animals throughout the European Union. No method is available to reliably detect the misuse of natural hormones in cattle. A method was developed to detect the abuse of testosterone in cattle fattening. Synthesized testosterone is rather depleted in the (13)C/(12)C ratio. Hence, the method is based on gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) analysis of urine. To select testosterone metabolites and endogenous reference compounds (ERC), the concentration of urinary steroids of cattle was investigated. Dehydroepiandrosterone and androst-5ene-3beta,17alpha-diol were chosen as ERCs to show endogenous (13)C/(12)C ratios. Etiocholanolone and 5alpha-androstane-3beta,17alpha-diol were chosen as the most important testosterone metabolites. Other metabolites known from literature like epitestosterone were less promising. In principle, GC/C/IRMS is a nonspecific method because finally carbon dioxide is analyzed. Therefore, a dedicated cleanup procedure for the selected steroids was developed. By means of proposed confidence intervals in the isotopic composition of ERCs and metabolites, the administration of testosterone to cattle could be detected reliably. Differences of up to 11 per thousand on the delta-scale between ERC and testosterone metabolites were found after testosterone administration, whereas endogenous differences did not exceed 2 per thousand.  相似文献   
173.
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